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And Ts’o, P. O. P. (1986) In vitro senescence of syrian hamster mesenchymal cells of fetal to aged adult origin, inverse relationship between in vivo donor age and in vitro proliferative capacity. Mech. Ageing Dev. 34, 151–173. 68. , Moerman, E. , Soeldner, J. , Gleason, R. , and Barnett, D. M. (1978) Chronologic and physiologic age affect replicative life-span of fibroblasts from diabetic, prediabetic, and normal donors. Science 199, 781–782. 69. Schneider, E. L. (1979) Cell replication and aging: in vitro and in vivo studies.

133, 135–143. 52. , Cristofalo, V. , Ozer, H. , Roberts, C. , LeRoith, D. (1993) Failure of senescent human fibroblasts to express the insulin-like growth factor-1 gene. J. Biol. Chem. 268, 17,883–17,888. 53. Brooks-Frederich, K. , Cianciarulo, F. , Rittling, S. , and Cristofalo, V. J. (1993) Cell cycle-dependent regulation of Ca2+ in young and senescent WI-38 cells. Exp. Cell Res. 205, 412–415. 54. Allen, R. , Keogh, B. , Doggett, D. , and Cristofalo, V. J. (1999) Differences in electron transport potential antioxidant defenses and oxidant generation in young and senescent fetal lung fibroblasts (WI-38).

182, 315–321. 99. Praeger, F. C. and Cristofalo, V. J. (1986) The growth of WI-38 in a serum-free, growth factor-free, medium with elevated calcium concentrations. In Vitro 22, 355–359. 100. Furth, J. , Allen, R. , and Cristofalo, V. J. (1997) Abundance of α1(I) and α1(III) procollagen and p21 mRNAs in fibroblasts cultured from fetal and postnatal donors. Mech. Ageing Dev. 97, 131–142. 101. , Robertorye, R. , Adami, G. , Pereira-Smith, O. , and Smith, J. R. (1995) Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1.

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Aging Methods and Protocols (Methods in Molecular Medicine) by Yvonne A. Barnett

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