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Read e-book online Amino acids analysis Protocols PDF

By Catherine Cooper

ISBN-10: 0896036561

ISBN-13: 9780896036567

ISBN-10: 1592590470

ISBN-13: 9781592590476

A suite of vintage and state-of-the-art suggestions of excessive application in answering particular organic questions on amino acids. universal tools contain these in response to HPLC or fuel chromatography separation and research after precolumn derivatization. New suggestions in response to capillary electrophoresis separation, high-performance anion alternate chromatography, and mass spectrometry also are awarded. every one process is defined in step by step aspect to make sure winning experimental effects and emphasizes pattern practise, fairly the gathering and garage of physically fluids. up to date and hugely sensible, Amino Acid research Protocols bargains analytical and scientific chemists, in addition to a vast diversity of organic and biomedical investigators, a wealthy compendium of laboratory instruments for the efficient research of either universal and unusual amino acids.

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5. Soybean powder: Chromatographic results from three different hydrolytic procedures. 6. Screw down the Teflon plug to seal the vacuum inside the tube. 7. Place the sealed tube into a preheated Reacti-Therm Module, and hydrolyze (see Note 7 for temperature setting and timing of the respective hydrolytic protocols). 8. Remove the tube from the heating module and allow it to cool before breaking the vacuum inside the tube. 9. Transfer the hydrolysate into a clean microcentrifuge tube. 10. Carry out evaporative centrifugation (see Note 10).

031481, Notes 15–17). 2. 5-mL autosampler vials (see Notes 18–20). Place vials into the autosampler, note their positions. 3. 5-mL vial with 8 µM solution of hydrolysate standard. 5mL vial with water. Place vials into the autosampler, note their positions. 4. Write a PeakNet Schedule (see PeakNet Software User’s Guide, Dionex Document No. 0-µL full-loop injections and assigning the gradient pump method from Table 2 together with the detection method from Table 5 to each of the samples and standards.

4. HPLC/spectroscopic grade triethylamine, methanol, acetone, and dimethyl sulfoxide (DMSO) from Fluka. 3. Chromatographic Analysis 1. Solvent delivery system: Any typical HPLC system available from a number of manufacturers can be used for resolution of derivatized amino acids. We have recently used HPLC systems from Bio-Rad (HRLC 800 equipped with an autoinjector AST 100), Hewlett Packard 1050 system and Waters system. 2. 6 mm; 5 µm from The Sep/a/ra/tions group). 3. , Bio-Rad 1790 UV/VIS monitor, Hewlett Packard Photodiode Array 1050, Waters Photodiode Array 996).

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Amino acids analysis Protocols by Catherine Cooper

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