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Angiotensin Protocols - download pdf or read online

By Donna H. Wang

ISBN-10: 1617371769

ISBN-13: 9781617371769

Michigan nation Univ., East Lansing. interprets wisdom into reproducible equipment than let biomedical researchers to discover the function of angiotensin in wellbeing and fitness and disorder. Covers genetic manipulation of unique genes. comprises tools for sensible reports of the renin-angiotensin process. DNLM: Renin - Angiotensin System-physiology

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161, 430–439. 30. , et al. (1999) Interaction of angiotensin II and mechanical stretch on vascular endothelial growth factor production by human mesangial cells. J. Am. Soc. Nephrol. 10, 730–737. 31. Wilson, H. , Haites, N. , and Booth, N. A. (1997) Effect of angiotensin II on plasminogen activator inhibitor-1 production by cultured human mesangial cells. Nephron 77, 197–204. 32. , and Iwao, H. (1998) Activation of glomerular mitogen-activated protein kinases in angiotensin II-mediated hypertension.

24) used a gene-targeting knock-in procedure to insert the cre-recombinase gene downstream of the MLC-2v promoter in the genome. They did this assuming that all regulatory elements needed for correct targeting of ventricular myocytes would be present if crerecombinase were inserted directly into the genome downstream of the promoter.

In other genes, exactly which exon or exons are chosen will depend upon the protein in question. However, it may be attractive to try to identify a region of the protein that is critical for its function and flox the exons encoding that region. Such motifs may include those signaling secretion or intracellular trafficking, interactions with other proteins or DNA, membrane spanning domains, and active sites of enzymes (see Note 1). 60 Sigmund and Stec Fig. 5. Schematic of targeting vectors. A schematic representation of a standard targeting (A) or floxing (B) vector is shown.

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Angiotensin Protocols by Donna H. Wang

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