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Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N.'s Applications of Chimeric Genes and Hybrid Proteins - Part C: PDF

By Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N. Abelson

ISBN-10: 012182229X

ISBN-13: 9780121822293

The significantly acclaimed laboratory typical for greater than 40 years, Methods in Enzymology is without doubt one of the such a lot hugely revered courses within the box of biochemistry. because 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless appropriate today--truly an important book for researchers in all fields of existence sciences

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Additional info for Applications of Chimeric Genes and Hybrid Proteins - Part C: Protein-Protein Interactions and Genomics

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3-7. Oxford University Press, Oxford, 1997. 5 R. Brent and R. L. , Annu. Rev. Genet. 31, 663 (1997). 6 R. L. Finley, Jr. and R. Brent, Proc. Natl. Acad. Sci. A. 91, 12980 (1994). 7 C. Bendixen, S. Gangloff, and R. Rothstein, Nucleic Acids Res. 22, 1778 (1994). a HIS3 HIS3 A D H l p - L e x A (DBD) A D H l p - L e x A (DBD) 1 5 TRP1 URA3 GALlp-B42 (AD) ADHlp-B42 (AD) 6 Unpublished URA3 L YS2 8LexAop-lacZ 8LexAop-lacZ 5 7 a Key to references: (1) J. Estojak, R. Brent, and E. A. Golemis, Mol. Cell.

Sci. A. 88, 9578 (1991). 328 Copyright© 2000by AcademicPress All rightsof reproductionin any formreserved. 00 [9,] L e x A - B A S E D TWO-HYBRID SYSTEMS 15 TABLE I COMPARISON OF PLASMIDS AND STRAINS FOR USE IN LexA Two-HYBRID SYSTEMSa Characteristic Basic bait plasmids Typical representative Yeast markers Bacterial markers Expression Alternative bait plasmids NLS enhanced Inducible expression COOH-terminal LexA Reporters I. Auxotrophic Typical representative Sensitivity regulation Yeast markers 2.

And II separately, underscoring the strengths and weaknesses of each approach at the end of the chapter. LexA System I In the LexA system I, 3 the selective reporter contains lexA operators upstream of LEU2, a gene required in the yeast leucine biosynthetic pathway. A number of yeast strains into which the lexAop-LEU2 reporter gene has been chromosomally integrated are available. The two most commonly used strains are EGY48 and EGY191, which provide differential sensitivity for trans-activation.

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Applications of Chimeric Genes and Hybrid Proteins - Part C: Protein-Protein Interactions and Genomics by Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N. Abelson


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